APPENDIX 1
Equipment List
- Spectrophotometer (Unicam UV4-100 UV/visible light)
- Six tube centrifuge (Gallenkamp Junior)
- Twelve tube microcentrifuge (Clements Orbital 100)
- Water bath (Labmaster)
- PCR thermocycler (Corbett Research FTS-320 Fast Thermal Sequencer)
- Power Supply (Pharmacia Electrophoresis Constant Power Supply ECPS 3000/150)
- Small electrophoresis buffer tank (for agarose gels)
- Large electrophoresis aparatus (Hoefer Scientific Instruments, for polyacrylamide gels)
- UV Transilluminator (Hoefer Scientific Instruments) and Polaroid Film 667
Chemicals
- All chemicals used were analytical grade reagents
- Acetic acid - BDH Chemicals
- Acrylamide - BDH Chemicals
- Agarose (electrophoresis grade) - Pharmacia
- APS (ammonium persulphate) - Pharmacia Biotech
- Bis-acrylamide (NN'-Methylenebisacrylamide) - BDH Chemicals
- Boric acid - BDH Chemicals
- Bromophenol blue
- Detergent (Decon 90) - Rhone Poulenc
- EDTA (ethylenediamine tetracetic acid; disodium salt: dihydrate) - Sigma
- Ethanol (95%)
- Ethidium bromide - Sigma
- Formaldehyde - BDH Chemicals
- Glycerol
- Hydrochloric acid - Univar
- Milli-Q Water
- Mineral Oil - Sigma
- NaCl (sodium chloride) - BDH Chemicals
- NaOH (sodium hydroxide) - BDH Chemicals
- Nitric acid - Univar
- SDS (sodium dodecyl sulfate) - Sigma
- Silver nitrate - BDH Chemicals
- Sodium carbonate - Univar
- TEMED (N,N,N',N'-Tetramethylethylenediamine) - Pharmacia Biotech
- Tris Base (Tris[hydroxymethyl]aminomethane) - Sigma
- Tris-HCl (Tris[hydroxymethyl]aminomethane hydrochloride) - Sigma
- Xylene cyanol
Enzymes
- Proteinase K - Merck
- Taq DNA polymerase and 10x reaction buffer - Boehringer Mannheim; Pharmacia Biotech
- TaqI restriction enzyme and 10x reaction buffer - Progen
Molecular Size Marker
The molecular size marker used for checking DNA fragment sizes
was purified pUC19 DNA digested with Hpa II, purchased
from Progen Industries Ltd., Darra, QLD. Fragment sizes are:
| 1) 501 | 7) 147 |
| 2) 489 | 8) 111 |
| 3) 404 | 9) 110 |
| 4) 331 | 10) 67 |
| 5) 242 | 11) 34 |
| 6) 190 | 12) 26 |